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wurster's blue tmpd oxidised phenylenediamine electron oxidation -tetramethyl-p-phenylenediamine radical cation blue-violet redox indicator oxidase test cytochrome tetramethylphenylenediamine redox aqueous solution titration 3d substance matter material compound component agent stuff reagent functional group precursor synthesis reaction production building block bioactive cell biochemistry biology biomolecule molecule molecular formula skeletal structural structure geometry ball stick chemical science scientific research organic