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mes 2-morpholin-4-ylethanesulfonic acid zwitterion 2-morpholin-4-ium-4-ylethanesulfonate morpholine ring mops propanesulfonic buffering agent biology biochemistry melting point range mes buffer solution tris buffer good's buffers solvent coordination complexes safety hazard toxic rendering ph hydrogen ion capacity mixture base substance matter material compound component stuff reagent functional group synthesis reaction production building block